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Test Code LAB10111 Urea Nitrogen - Urine

Department

CHEMISTRY

Primary Collection Container

Yellow Urine Vacutainer (No Additive)

Alternate Collection Container

Sterile Container

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Keep specimen refrigerated until processing.

Centrifuge and refrigerate.

Stability

Ambient: 8 hours

Refrigerated: 1 week

Frozen: Unacceptable

Minimum Testing Volume

0.1 mL

Reference Range

Not established

Methodology

Urease specifically hydrolyzes urea to form ammonia and carbon dioxide. The ammonia is used by the enzyme glutamate dehydrogenase (GLDH) to reductively aminate a-ketoglutarate (a-KG), with simultaneous oxidation of reduced nicotinamide-adenine dinucleotide (NADH). The change in absorbance at 340 nm due to the disappearance of NADH is directly proportional to the urea nitrogen concentration in the sample and is measured using a bichromatic (340, 383 nm) rate technique. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

84540