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Test Code LAB12000 Stool Culture - Outpatient

Department

MICROBIOLOGY

Collection Requirements

Collect fresh random stool or rectal swab.

Add stool specimen to transport until liquid reaches fill line.

Emulsify specimen thoroughly in transport fluid.

Send stool in transport media within 72 hrs of collection or rectal swab in aerobic transport within 24 hrs.

Do not refrigerate or incubate.

Lesions of the rectum or sigmoid colon may be swabbed during proctoscopy or sigmoidoscopy and are preferred to swabs blindly inserted into the rectum.

When multiple cultures ordered, submit one per day.

Primary Collection Container

Cary-Blair Media (Orange)

Alternate Collection Container

Gel Bottom Swab

Transport

Ambient

Preferred Transport Temperature

Ambient

Stability

Ambient:

Para-Pak C&S: 72 hours

Aerobic Gel Bottom Swab: 24 hours

Reference Range

No Salmonella, Shigella, Campylobacter, or E. Coli 0157:H7 Isolated

Methodology

Culture

Performed

Sunday – Saturday

Reported

4 days

CPT Codes

87045; 87046

Identification, and susceptability tests are billed separately from culture

Unacceptable Conditions

Delay in delivery to the lab.

Nonsterile or leaking containers, multiple specimens (more than one in 24 hours), dry specimens, or delayed transport without use of appropriate preservative. Refrigerated specimens.

Additional Information

Primary organisms that are always looked for: Salmonella, Shigella, Campylobcter, and E. coli O157:H7.

Vibrio sp. and Yersinia sp. are ordered separately and sent to a reference laboratory. Please search “stool culture, vibrio; stool culture, yersinia”, for order details. Ova and Parasite and Clostridium difficile are not recovered through routine culture.