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Test Code LAB12014 Acid Fast (AFB) Stain

Department

MICROBIOLOGY

Collection Requirements

Fresh early morning sputum specimens collected on successive days are preferred. Not to exceed 10 mL. Important that sputum expectoration be “from deep down in the lungs” as opposed to the expectoration of saliva or nasalpharyngeal secretions.

Other types of specimens that may be submitted for mycobacteria isolation are: Tissue, body fluids, urine, gastric, and stool.

Must indicate source.

Primary Collection Container

Sterile Container

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

None

Stability

Refrigerated: 24 hours

Minimum Testing Volume

2.0 mL

Reference Range

No Acid-Fast Bacilli Observed.

Methodology

Auramine/Rhodamine Method

Kinyoun Method

Performed

Sunday – Saturday

6:30 am – 3:00 pm

Reported

Within 24 hours

CPT Codes

87206

Unacceptable Conditions

Specimens appearing to be expectorated as saliva.

Specimens with inadequate volume.

Additional Information

Acid Fast Stain is part of a routine Acid Fast Culture.