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Test Code LAB12023 Acid Fast (AFB) Culture with Stain

Department

MICROBIOLOGY

Collection Requirements

Fresh early morning sputum specimens collected on successive days are preferred. Not to exceed 10 mL. Important that sputum expectoration be “from deep down in the lungs” as opposed to the expectoration of saliva or nasalpharyngeal secretions.

Other types of specimens that may be submitted for mycobacteria isolation are: Tissue, body fluids, urine, gastric, and stool.

Must indicate source.

Primary Collection Container

Sterile Container

Transport

Ambient

Must be received within 24 hours of collection.

Preferred Transport Temperature

Ambient

Processing

If a respiratory culture is ordered on the same specimen, set-up the respiratory culture immediately upon receipt, then refrigerate specimen for acid fast set-up on first shift.

Stability

Ambient: 24 hours

Minimum Testing Volume

2.0 mL

Reference Range

No Mycobacterium Species Isolated.

Methodology

Culture

Performed

Monday – Friday

6:00 am – 3:00 pm

Reported

6-7 weeks

CPT Codes

87116; 87206

Unacceptable Conditions

Specimens appearing to be expectorated as saliva.

Specimens with inadequate volume.