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HelpTest Code LAB12241 Herpes Simplex Virus 1 and 2 PCR, Qualitative
Department
SEND OUTS
Reference Lab Test Number
HSV12
Collection Requirements
Specimen source required.
Swab specimens (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
CSF and body fluids undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum)
Blood in EDTA (lavender top) tube. 2.0 mL (1.0 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum).
Bone Marrow in EDTA. 1.0 mL (0.5 mL minimum).
Refer To
Primary Collection Container
Special
Transport
Refrigerated
Preferred Transport Temperature
Refrigerated
Stability
Ambient (18-25°C): 3 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Do not freeze whole blood or bone marrow.
Minimum Testing Volume
See collection information.
Reference Range
Not Detected
Methodology
RT-PCR amplification and detection procedure
Performed
Monday – Saturday
Reported
3 Days
CPT Codes
87529 x2
Unacceptable Conditions
Rectal swabs preserved in formalin, SAF, or PVA.
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Stool samples
Serum
Urine
Additional Information
Alternate Specimen:
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.