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Test Code LAB12402 Cytomegalovirus PCR, Qualitative

Department

SEND OUTS

Reference Lab Test Number

CYPCR

Collection Requirements

Specimen source required

Requires dedicated sample

Swab specimens (ocular, throat, rectal) in viral transport medium.

Swabs in culturettes must be transferred to viral transport within 24 hours of collection.

Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).

Bronchoalveolar lavage/wash in sterile leak-proof container, 1.0 mL (0.5 mL minimum).

Sputum Undiluted in sterile leak-proof container, 1.0 mL (0.5 mL minimum)

CSF and body fluids undiluted in sterile leak-proof container, 1.0 mL (0.5 mL minimum)

Nasal washes in leak proof sterile container, 1.0 mL (0.5 mL minimum)

Urine undiluted in sterile leak-proof container, 2.0 mL (1.0 mL minimum)

Blood in EDTA (lavender top) tube, 2.0 mL (1.0 mL minimum)

EDTA plasma (lavender top), 1. mL (0.5 mL minimum)

Bone Marrow in EDTA, 1.0 mL (0.5 mL minimum)

Amniotic Fluid, 1.0 mL (0.5 mL minimum) in sterile leak-proof container

Primary Collection Container

Lavender 6 mL (K3EDTA)

Alternate Collection Container

Sterile Container

Sterile Cup

Swab

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Stability

Ambient (18-25°): 3 days

Refrigerated (2-8°C): 2 weeks

Frozen (-20°C): 2 weeks

Frozen (-70°C): 3 months

Minimum Testing Volume

See collection requirements.

Reference Range

Not detected

Methodology

This test utilizes the polymerase chain reaction to amplify a 60 base pair region of the cytomegalovirus (CMV) DNA polymerase gene. Real-time detection and quantification are used to determine the viral copy number. The analytical sensitivity of this assa

Performed

Monday – Saturday

Reported

3 Days

CPT Codes

87496

Unacceptable Conditions

Rectal swabs preserved in formalin, SAF, or PVA

Urine in Grey-Top (boric acid) urine Vacutainers

Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media

Specimens in bacteriological blood culture media

Dry swabs, calcium alginate swabs, and swabs in gel transports

Culturettes older than 24 hours

Stool

Serum

Specimens received in non-sterile or leaking containers will not be tested

Additional Information

Alternate Specimen:

The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.