Test Code LAB12402 Cytomegalovirus PCR, Qualitative
Department
SEND OUTS
Reference Lab Test Number
CYPCR
Collection Requirements
Specimen source required
Requires dedicated sample
Swab specimens (ocular, throat, rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in sterile leak-proof container, 1.0 mL (0.5 mL minimum).
Sputum Undiluted in sterile leak-proof container, 1.0 mL (0.5 mL minimum)
CSF and body fluids undiluted in sterile leak-proof container, 1.0 mL (0.5 mL minimum)
Nasal washes in leak proof sterile container, 1.0 mL (0.5 mL minimum)
Urine undiluted in sterile leak-proof container, 2.0 mL (1.0 mL minimum)
Blood in EDTA (lavender top) tube, 2.0 mL (1.0 mL minimum)
EDTA plasma (lavender top), 1. mL (0.5 mL minimum)
Bone Marrow in EDTA, 1.0 mL (0.5 mL minimum)
Amniotic Fluid, 1.0 mL (0.5 mL minimum) in sterile leak-proof container
Refer To
Warde Medical LaboratoryPrimary Collection Container
Lavender 6 mL (K3EDTA)
Alternate Collection Container
Sterile Container
Sterile Cup
Swab
Transport
Refrigerated
Preferred Transport Temperature
Refrigerated
Stability
Ambient (18-25°): 3 days
Refrigerated (2-8°C): 2 weeks
Frozen (-20°C): 2 weeks
Frozen (-70°C): 3 months
Minimum Testing Volume
See collection requirements.
Reference Range
Not detected
Methodology
This test utilizes the polymerase chain reaction to amplify a 60 base pair region of the cytomegalovirus (CMV) DNA polymerase gene. Real-time detection and quantification are used to determine the viral copy number. The analytical sensitivity of this assa
Performed
Monday – Saturday
Reported
3 Days
CPT Codes
87496
Unacceptable Conditions
Rectal swabs preserved in formalin, SAF, or PVA
Urine in Grey-Top (boric acid) urine Vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Stool
Serum
Specimens received in non-sterile or leaking containers will not be tested
Additional Information
Alternate Specimen:
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.