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Test Code LAB12435 Factor V Leiden (APCR) w/Reflex to Gene Mutation by PCR

Department

HEMATOLOGY

Collection Requirements

Must be a full draw.

Mix thoroughly immediately after collection.

Primary Collection Container

2 – Blue 2.7 mL (Citrate)

1 – Pink (K3EDTA)

Transport

Blue Top: Frozen

Pink (K3EDTA): Ambient

Preferred Transport Temperature

Blue Top: Frozen

Pink (K3EDTA): Ambient

Processing

Centrifuge Blue Top tube within 30 minutes of collection.

Centrifuge at 3300 rpm for 20 minutes.

Pink(K3EDTA) Transport as whole blood.

Stability

Blue Sodium Citrate Tube:

Ambient: Unacceptable

Refrigerated: 4 hours

Frozen: 30 days

Pink (K3EDTA):

Ambient: 5 days

Refrigerated: Not recommended.

Frozen: Unacceptable

Minimum Testing Volume

Blue Top: 1.0 mL

Pink(K3EDTA): 3.0 mL

Reference Range

By report

Methodology

Turbidimetric Clot Detection ACL Top PCR Osmetech

Performed

Monday, Wednesday, and Friday

7:00 am – 2:00 pm

Specimen must be received in Houghton lab by 1pm.

Reported

As completed

CPT Codes

85307; Add 81241 if reflexed at additional cost

Unacceptable Conditions

Hemolyzed, clotted, short drawn, non-frozen separated blue top tubes and serum specimens.