Sign in →

Test Code LAB12479 Bilirubin, Direct - Neonatal

Department

CHEMISTRY

Collection Requirements

ONLY FOR BABIES IN NICU OR OB FLOORS.

PROTECT FROM LIGHT.

Primary Collection Container

Green Microtainer PST

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Separate from cells if testing is delayed more then 8 hours.

Deliver to chemistry ASAP.

Stability

Refrigerated: 48 hours, when protected from light and separated from cells.

Minimum Testing Volume

0.1 mL

Reference Range

0-14 days <0.5 mg/dL

>14 days <0.5 mg/dL

Methodology

Diazotized sulfanilic acid is formed by combining sodium nitrite and sulfanilic acid at low pH. The sample is diluted in 0.5M HCl. A sample blank reading is taken to eliminate interference from non-bilirubin pigments. Upon addition of the diazotized sulfanilic acid, the conjugated bilirubin is converted to diazo-bilirubin, a red chromophore which absorbs at 540 nm and is measured using a bichromatic (540, 700 nm) endpoint technique. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

82248

Unacceptable Conditions

Exposure to light for extended periods.