Sign in →

Test Code LAB13020 Protein, Quantitative - 12 Hour Urine

Department

CHEMISTRY

Collection Requirements

12 hour urine.

Do not add preservatives to the collection container.

Keep the container on ice or refrigerated during collection.

Primary Collection Container

24 HR Urine container

Transport

Urine refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Keep specimen refrigerated until processing.

Measure total volume.

Aliquot, centrifuge and refrigerate.

Note total volume on the label.

Stability

Ambient: 8 hours

Refrigerated: 3 days

Minimum Testing Volume

1.0 mL

Reference Range

≤50 mg/12h

Methodology

In the reaction sequence, pyrogallol red combines with sodium molybdate to form a red complex with maximum absorbance at 470 nm. The protein in the sample reacts with this complex in acid solution to form a bluish-purple colored complex, which absorbs at 600 nm. The absorbance at 600 nm is directly proportional to the concentration of protein in the sample. The analyte concentration is determined by calculation using a logit curve fit on a previously stored calibration curve. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

84156

Unacceptable Conditions

Frozen specimen.

Grossly bloody Specimen.