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Test Code LAB13085 Bilirubin, Total - Body Fluid

Department

CHEMISTRY

Collection Requirements

PROTECT FROM LIGHT.

Specimen Source Required.

Primary Collection Container

Sterile Container

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Stability

Ambient: 4 hours (protected from light)

Refrigerated: 5 days

Minimum Testing Volume

1.0 mL

Reference Range

No Reference Ranges have been established.

Methodology

Diazotized sulfanilic acid is formed by combining sodium nitrite and sulfanilic acid at low pH. Bilirubin (unconjugated) in the sample is solubilized by dilution in a mixture of caffeine/benzoate/acetate/EDTA. Upon addition of the diazotized sulfanilic acid, the solubilized bilirubin including conjugated bilirubins (mono and diglucoronides) and the delta form (biliprotein-bilirubin covalently bound to albumin) is converted to diazo-bilirubin, a red chromophore representing the total bilirubin which absorbs at 540 nm and is measured using a bichromatic (540, 700 nm) endpoint technique. Siemens Dimension Vista and ExL.

Performed

Monday – Sunday

Reported

As completed

CPT Codes

82247

Unacceptable Conditions

Specimen stored at room temperature.

Gross Hemolysis.

Highly Viscous Fluid.

Additional Information

Testing for this specimen type has not been validated or FDA approved for this analyte. The test result must be integrated into the clinical context for interpretation.