Sign in →

Test Code LAB141 Uric Acid - Plasma

Department

CHEMISTRY

Primary Collection Container

Green PST (Lithium Heparin)

Alternate Collection Container

Green Microtainer PST

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Separate from cells within 2 hours.

Stability

Ambient: 8 hours

Refrigerated: 5 days

Frozen: 6 months

Minimum Testing Volume

0.1 mL

Reference Range

Male

0-14 yrs: 2.0 – 5.3 mg/dL >14 yrs: 3.2 – 6.6 mg/dL

Female

0-14 yrs: 2.0 – 5.3 mg/dL >14 yrs: 2.3 – 5.0 mg/dL

Methodology

The uric acid method is a modification of the uricase method first reported by Bulger and Johns, later modified by Kalckar. Uric acid, which absorbs light at 293 nm, is converted by uricase to allantoin, which is nonabsorbing at 293 nm. The change in absorbance at 293 nm due to the disappearance of uric acid is directly proportional to the concentration of uric acid in the sample and is measured using a bichromatic (293, 700 nm) endpoint technique. Measurement of uric acid by monitoring the loss of absorbance at 293 nm following uricase treatment is generally recognized as being more specific and less subject to interference than other indirect methods. Siemens Diemension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

84550