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HelpTest Code LAB188 Lactate Dehydrogenase (LDH) - Body Fluid
Department
CHEMISTRY
Collection Requirements
Specimen Source Required.
Primary Collection Container
Sterile Container
Transport
Ambient
Preferred Transport Temperature
Ambient
Processing
Deliver to lab ASAP.
Centrifuge and separate from cells within 2 hours.
Stability
Ambient: 24 hours
Refrigerated or Frozen specimens are not recommended.
Minimum Testing Volume
0.1 mL
Reference Range
No reference ranges have been established.
Methodology
The LDI method uses as a substrate L-lactate buffered at a pH of 9.4. Lactate dehydrogenase oxidizes the substrate in the presence of NAD+ to yield pyruvate and NADH which absorbs light at 340 nm. Lactate dehydrogenase activity is measured as a rate reaction at 340/700 nm, proportional to the amount of lactate dehydrogenase in the sample. Siemens Dimension Vista.
Performed
Sunday – Saturday
Reported
As completed
CPT Codes
83615
Unacceptable Conditions
Hemolysis.
Prolonged contact of the body fluid with separated red cells.
Additional Information
Testing for this specimen type has not been validated or FDA approved for this analyte. The test result must be integrated into the clinical context for interpretation.