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Test Code LAB196 Protein, Total - Body Fluid

Department

CHEMISTRY

Collection Requirements

Specimen Source Required.

Primary Collection Container

Sterile Container

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Stability

Refrigerated: 48 hours

Minimum Testing Volume

0.1 ml fliud

Reference Range

No reference ranges have been established.

Methodology

The total protein method is a modification of the biuret reaction first introduced by Kingsley and later modified by Henry and presented as the method of choice for serum by Henry. This method incorporates tartrate as a complexing agent to prevent precipitation of Cu(OH)2. Serum blanking increases method sensitivity and minimizes spectral interference from lipemia. Cupric ion (Cu++) reacts with the peptide linkages of protein in a basic solution. The blue copper (II) protein complex thus formed is proportional to the total protein concentration in the sample and is measured using a bichromatic (540, 700 nm) endpoint technique. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

84157

Unacceptable Conditions

Specimen stored at room temperature.

Gross hemolysis.

Additional Information

Testing for this specimen type has not been validated or FDA approved for this analyte. The test result must be inegrated into the clinical context for interpretation.