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Test Code LAB20051 Glucose Tolerance, 2 Hour - Pediatric

Department

CHEMISTRY

Collection Requirements

This is the standard 2 hour glucose tolerance test used in the diagnosis of diabetes mellitus in children less than 12 years old.

8 hour fast is required

Patient should be on a high carbohydrate diet for 72 hours prior to the test.

Draw the fasting glucose as soon as possible after patient’s arrival.

The prepared dextrose dose must be calculated in patients less than 94 lbs. If starting with a standard size prepared dextrose bottle of 100gms/10oz, then the calculation is as follows: Patient weight in lbs. X 0.08 = ounces of prepared dextrose. Maximum prepared dextrose dose is 75gm (7.5oz.). Record Grams of dextrose given on Tolerance worksheet, Oz of glucola x 10 = Grams. If using a non-standard size bottle of prepared dextrose, phone the Chemistry Section (989-583-6782) for help in calculating the proper dose.

Verify each collection for the exact draw time. Do not accept default draw times.

Primary Collection Container

Green PST (Lithium Heparin)

Alternate Collection Container

Green Microtainer PST

Transport

Plasma refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Separate from cells if testing is delayed more than 2 hours. Deliver to Chemistry ASAP.

Stability

Refrigerated: 72 hours

Minimum Testing Volume

0.5 mL

Reference Range

By Report

Methodology

Hexokinase (HK) Roche c503

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

82951

Unacceptable Conditions

Gross hemolysis.

Delay in separating cells from plasma.

Additional Information

Test includes: Fasting, 1 hour and 2 hour glucose, and an interpretation guideline for the diagnosis of Diabetes Mellitus.