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Test Code LAB20352 Pertussis PCR Panel

Department

Send Outs

Reference Lab Test Number

BPPCR

Collection Requirements

Specimen source required

Primary Collection Container

Viral Transport UTM

Transport

Nasopharyngeal Swab Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Collect nasopharyngeal swab (Dacron or Rayon swabs only), place in viral transport media (M4RT).

Stability

Room Temperature: 3 days

Refrigerated: 14 days

Frozen (-20°C): 14 days

Frozen (-70°C): 3 months

Reference Range

Not detected

Methodology

This Pertussis Panel utilizes the multi-target detection approach recommended by the Centers for Disease Control and Prevention (MMWR 2007; 56(33):837). This panel includes real-time PCR testing for the IS481 gene of B. pertussis, the p1S1001 gene from B. parapertussis, and the h1S1001 gene from B. holmseii. Reflex testing for the pertussis toxin gene is used as needed to detect dual infections with B. parapertussis and B. holmseii. The analytical sensitivity of this panel is approximately 3 colony forming units/assay.

Performed

Monday-Saturday

Reported

3 Days

CPT Codes

87798 × 3

Unacceptable Conditions

Specimens in other transport media

Specimens in Regan Lowe media

Specimens in bacteriological blood culture media

Dry swabs, calcium alginate swabs, wooden swabs and swabs in gel transports

Specimens in culturettes

Throat and anterior nasal swabs (due to unacceptably low rates of detection)

Specimen received in non-sterile or leaking containers.