Test Code LAB20352 Pertussis PCR Panel
Department
Send Outs
Reference Lab Test Number
BPPCR
Collection Requirements
Specimen source required
Refer To
Primary Collection Container
Viral Transport UTM
Transport
Nasopharyngeal Swab Refrigerated
Preferred Transport Temperature
Refrigerated
Processing
Collect nasopharyngeal swab (Dacron or Rayon swabs only), place in viral transport media (M4RT).
Stability
Room Temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Reference Range
Not detected
Methodology
This Pertussis Panel utilizes the multi-target detection approach recommended by the Centers for Disease Control and Prevention (MMWR 2007; 56(33):837). This panel includes real-time PCR testing for the IS481 gene of B. pertussis, the p1S1001 gene from B. parapertussis, and the h1S1001 gene from B. holmseii. Reflex testing for the pertussis toxin gene is used as needed to detect dual infections with B. parapertussis and B. holmseii. The analytical sensitivity of this panel is approximately 3 colony forming units/assay.
Performed
Monday-Saturday
Reported
3 Days
CPT Codes
87798 × 3
Unacceptable Conditions
Specimens in other transport media
Specimens in Regan Lowe media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, wooden swabs and swabs in gel transports
Specimens in culturettes
Throat and anterior nasal swabs (due to unacceptably low rates of detection)
Specimen received in non-sterile or leaking containers.