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Test Code LAB20422 SARS-Cov-2 Total Antibody Screen

Important Note

This test includes both the spike total antibody and the nucleocapsid total antibody to SARS-CoV-2



Collection Requirements

Suggest to be drawn greater than 14 days post exposure, onset of symptoms or post vaccination.

Primary Collection Container

Gold (SST)

Alternate Collection Container

Yellow Microtainer SST


Refrigerated up to 7 days

Preferred Transport Temperature



Centrifuge and refrigerate


Refrigerated: 7 days

Minimum Testing Volume

2 mL

Reference Range





Mon-Fri 6 AM-2:30 PM


3-5 days

CPT Codes


Unacceptable Conditions

Grossly hemolyzed. Grossly Lipemic

Additional Information

SARS-CoV-2 Total Spike Antibody – This test will detect antibodies developed due to prior or current infection and will also likely detect antibodies against spike glycoprotein of SARS-CoV-2 generated following vaccination. This test will not differentiate between the 2 events. The absence of antibodies in this assay does not rule out recent infection.

SARS-CoV-2 Total Nucleocapsid Antibody – This test provides qualitative detection of serum antibodies against the nucleocapsid protein of SARS-CoV-2 indicating recent or prior infection. This test will not yield a positive result following vaccination against SARS-CoV-2.




Healthcare Providers Fact Sheet

For Recipients Fact Sheet



Healthcare Providers Fact Sheet

For Recipients Fact Sheet


Serology Test Results

Negative or Non-Reactive Test results:

• A Negative or Non-Reactive result indicates there are no detectable antibodies to SARS-CoV-2.

• Negative Antibody results do not rule out current or recent SARS-CoV-2 infection. If SARS-CoV-2 infection is suspected follow up with Molecular PCR testing for Covid-19.

• False-negative Antibody results may occur in serum collected too soon after symptom onset or exposure. Typically, the majority of patients seroconvert within 1 to 3 weeks.

• Over time, antibody titers may decline and eventually become negative.

• A negative result may be found in patients with a depressed immune response including the elderly, immunocompromised or immunosuppressed patient.


Positive or Reactive Test Results:

• A Positive or Reactive result indicates that antibodies were detected to SARS-CoV-2.

• A Positive or Reactive results indicates likely past exposure or past infection by SARS-CoV-2.

• Positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E.

• Protective immunity cannot be inferred based on these results alone.

• The duration and relative amount of antibodies remaining in the blood following COVID-19 is unknown.

• Positive Serological results should not be used to diagnose recent SARS-CoV-2 Infection; If SARS-CoV-2 infection or recent exposure is suspected follow up with Molecular PCR testing