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Test Code LAB20473 Influenza Virus A and B PCR - Sputum/Bronch Lavage

Department

SEND OUTS

Reference Lab Test Number

FLPCR

Collection Requirements

Specimen source is required.

NP and Throat swabs in viral transport medium.

If sending one sample for FLCPR and COVW (COVID testing) please send a minimun of 1.5 mL.

Swabs in culturettes must be transferred to viral transport within 24 hours of collection.

Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container 1.0 mL (0.5 mL minimum).

Sputum undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).

Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).

Nasal washes in an IATA-approved sterile screw-capped plastic container 1.0 mL (0.5 mL minimum).

NP/OP swab in Phosphate Buffered Saline or Sterile Saline.

Refer To

Warde Medical Laboratory

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Stability

Ambient: 3 days

Refrigerated: 14 days

Frozen (-20°C): 14 days

Frozen (-70°C): 3 months

Reference Range

Not detected

Methodology

Influenza A and Influenza B virus detecton is performed by reverse transcription of Influenza A and Influenza B RNA sequences and real-time PCR amplification and detection. The Influenza A assay detects the presence of a conserved region of the matrix gene and the Influenza B assay detects a conserved region of the NS1 protein. Available nucleic acid sequence information suggest that the Influenza A test will detect all known Influenza A subtypes including avian Influenzas. However, this method cannot distinguish avian from non-avian subtypes. The analytical sensitivity of this assay is 100 copies/mL.

Performed

Monday – Saturday

Reported

2-4 days

CPT Codes

-87502

Unacceptable Conditions

Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.

Specimens in bacteriological blood culture media.

Dry swabs, calcium alginate swabs, and swabs in gel transports.

Culturette specimens older than 24 hours.

Specimens received in non-sterile or leaking containers.

Whole blood

Serum

Plasma