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HelpTest Code LAB20586 BK Virus DNA PCR, Quantitative, Urine
Department
SEND OUTS
Reference Lab Test Number
UBKQN
Collection Requirements
Collect random urine.
Dedicated specimens are required. Specimens used in other assays will not be tested.
Refer To
Warde Medical LaboratoryPrimary Collection Container
Sterile Container
Transport
Undiluted urine frozen
Preferred Transport Temperature
Frozen
Processing
Send 2.0 mL urine undiluted and frozen in a sterile screw capped plastic container. Dedicated specimens are required. Specimens used in other assays will not be tested.
Stability
Ambient: 8 hours
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months
Minimum Testing Volume
1.0 mL
Reference Range
BKV QUAL: Not detected (NOT DET)
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL
Methodology
This test utilizes a polymerase chain reaction to amplify sequences from the VP1 regions of the BK virus genome. Real-time detection & quantification are used to determine the viral copy number. The analytical measurement range is 125 to 5 million copies/mL with an extended range up to 50 billion copies/mL with dilution (2.10 to 10.70 log(10 copies/mL). The qualitative limit to detection is 100 copies/mL (2.00 log(10) copies/mL). Specimens reported as “DETECTED” byt <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A “Not detected” result does not rule out infection.
Performed
Monday – Friday
Reported
4 days
CPT Codes
87799
Unacceptable Conditions
Shared specimens, specimens submitted to repeated freeze-thaw cycles, urine in Grey top (boric acid) urine Vacutainers, specimens received in non-sterile or leaking containers, specimens that do not meet the storage/handling conditions criteria above