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Test Code LAB20587 Adenovirus PCR

Department

SEND OUTS

Reference Lab Test Number

AVPCR

Collection Requirements

Specimen source required.

Swabs (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.

Swabs in culturettes must be transferred to viral transport within 24 hours of collection.

Biopsy/tissue specimens in saline or viral transport medium (Snap frozen-20°C).

Bronchoalveolar lavage/wash in sterile IATA-approved screw-capped plastic container 1.0 mL.

CSF and body fluid undiluted in sterile IATA-approved screw-capped plastic container. 1.0 mL.

Nasal aspirated in vacuum trap. 1.0 mL.

Nasal washes in IATA-approved screw-capped plastic container. 1.0ml.

EDTA plasma (lavender top). 1.0 mL.

Bone marrow in EDTA (lavender top) 1.0 mL.

Urine undiluted in an IATA-approved screw-capped plastic container. 2.0 mL.

Stool in sterile IATA-approved screw-capped plastic container 2.0 mL for liquid stool or marble -size solid stool.

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Stability

Ambient: 3 days

Refrigerated: 14 days

Frozen (-20°C): 14 days

Frozen (-70°C): 3 months

Reference Range

Not Detected

Methodology

Polymerase chain reaction (PCR). This method is genus specific and cannot distinguish adenoviral subtypes. The analytical sensitivity of this assay is 200 DNA copies/mL.

Performed

Monday – Saturday

Reported

3-4 days

CPT Codes

-87798

Unacceptable Conditions

Rectal swabs preserved in formalin, SAF, or PVA

Urine in Grey-Top (boric acid) urine vacutainers

Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.

Specimens in bacteriological blood culture media.

Dry swabs, calcium alg.

Culturettes older than 24 hours.

Specimens received in non-sterile or leaking containers.

Serum.

Whole blood.