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Test Code LAB20643 Culture Referred for Identification, Fungus

Important Note

Covenant Laboratory Only Order

Infectious

Reporting Name

Culture Referred for ID, Fungus

Useful For

Identification of pure isolates of filamentous fungi and yeast

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


1. See Infectious Specimen Shipping Guidelines for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.



Necessary Information


1. Specimen source (anatomic body site) is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.



Specimen Required


Specimen Type: Yeast or filamentous fungus in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube:

Preferred: Sabouraud dextrose agar slant

Acceptable: Inhibitory mold agar slant

Specimen Volume: Isolated mold or yeast

Collection Instructions: Yeast or filamentous fungus must be in pure culture, actively growing. Do not submit mixed cultures.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) Performed

Monday through Saturday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87107-Culture, fungi, definitive identification

87106-Culture, fungi, definitive identification, each organism; yeast (if appropriate)

87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107-Culture, fungi, definitive identification, each organism; mold (if appropriate)

87107-Fungal identification Panel A (if appropriate)

87107-Fungal identification Panel B (if appropriate)

87153-D2 fungal sequencing Identification (if appropriate)

87153-ITS Fungal Sequencing (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FUNID Culture Referred for ID, Fungus 42804-5

 

Result ID Test Result Name Result LOINC Value
FUNID Culture Referred for ID, Fungus In Process

Clinical Information

Fida M, Wengenack NL, Theel ES. Mycology: General approaches for direct and indirect detection and identification of fungi. In: Carroll KC, Pfaller MA, Pritt BS, et al. Manual of Clinical Microbiology. 13th ed. ASM Press; 2023

Interpretation

Genus and species are reported on fungal isolates whenever possible.

Method Description

Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and/or D2 and internal transcribed spacer (ITS) rDNA gene sequencing, as applicable.(Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL. Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011;49[4]:1614-1616; Hall L, Wohlfiel SL, Roberts GD. Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004;42[2]:622-626; Theel ES, Schmidt BH, Hall L, et al. Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012;50[9]:3093-3095; Theel ES, Hall L, Mandrekar J, Wengenack NL. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011;49[12]:4067-4071; General approaches for direct and indirect detection and identification of fungi. In: Carroll KC, Pfaller MA, Pritt BS, et al. Manual of Clinical Microbiology. 13th ed. ASM Press; 2023)

Report Available

2 to 35 days

Reject Due To

Agar plate Reject
 

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
D2F D2 Fungal Sequencing Identification No, (Bill Only) No
FUNA Fungal Ident Panel A No, (Bill Only) No
FUNB Fungal Ident Panel B No, (Bill Only) No
ITSF ITS Fungal Sequencing No, (Bill Only) No
RMALF Id MALDI-TOF Mass Spec Fungi No, (Bill Only) No
RMALY Id MALDI-TOF Mass Spec Yeast No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged. All fungal organisms submitted will be identified and billed as appropriate.

Method Name

Macroscopic/Microscopic/D2 and Internal Transcribed Spacer (ITS) rDNA Gene Sequencing/Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Dimorphic Pathogen Identification Confirmation: D2 and ITS rDNA Gene Sequencing/MALDI-TOF MS

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.