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Test Code LAB20716 Nicotine and Cotinine, Serum

Department

Send Outs

Reference Lab Test Number

SNICO

Primary Collection Container

Red (No Gel)

Transport

Serum Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge, separate serum from cells and send in a screw capped plastic vial.

Stability

Room temperature: 24 hours

Refrigerated: 7 days

Frozen: 14 days

Minimum Testing Volume

1.0 mL

Reference Range

                 Active Tobacco       User Passive        Exposure Abstinence 2 weeks and more
Nicotine   30 – 50 ng/mL          <2 ng/mL             <2 ng/mL
Cotinine   200 – 800 ng/mL      <8 ng/mL             <2 ng/mL

Methodology

High Performance Liquid

Chromatography/Tandem Mass Spectrometry

Performed

Monday, Wednesday, Friday

Reported

3-4 days

CPT Codes

G0480 (80323)

Unacceptable Conditions

Serum separator tube (SST), sodium citrate, grossly lipemic, hemolyzed, and icteric specimens.