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Test Code LAB324 Thrombin Time

Department

HEMATOLOGY

Collection Requirements

Blue top tube, must be a full draw.

Mix thoroughly after collection (immediately).

Must be received in coagulation within 4 hours.

Primary Collection Container

Blue 2.7 mL (Citrate)

Transport

Platelet-poor plasma on ice

Preferred Transport Temperature

Frozen

Processing

Mix tube thoroughly before centrifugation.

If freezing, prepare using the following method:

Centrifuge specimen. Use a plastic pipette to transfer top three-quarters of plasma into labeled plastic tube. When removing the plasma layer, be careful not to disturb the cell layers. Plasma separated from cells goes back into the centrifuge for a second spin. After the second spin, use a pipette again to transfer plasma to a new set of labeled aliquot tubes, then freeze.

Preparation of Platelet-Poor Plasma

Stability

Ambient: 2 Hours

Refrigerated: 4 Hours

Frozen: 2 Weeks

Minimum Testing Volume

1mL

Reference Range

15-22 sec

Methodology

Turbidimetric clot detection.

ACL Advance

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

85670

Unacceptable Conditions

Hemolyzed samples.

Clotted samples.

Short draw samples.

Serum specimens.