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Test Code LAB439 Protein, Quantitative - Urine

Department

CHEMISTRY

Collection Requirements

Random urine.

Primary Collection Container

Yellow Urine Vacutainer (No Additive)

Transport

Urine refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Stability

Ambient: 8 hours

Refrigerated: 3 days

Minimum Testing Volume

1.0 mL

Reference Range

No reference ranges have been established.

Methodology

In the reaction sequence, pyrogallol red combines with sodium molybdate to form a red complex with maximum absorbance at 470 nm. The protein in the sample reacts with this complex in acid solution to form a bluish-purple colored complex, which absorbs at 600 nm. The absorbance at 600 nm is directly proportional to the concentration of protein in the sample. The analyte concentration is determined by calculation using a logit curve fit on a previously stored calibration curve. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

84156

Unacceptable Conditions

Frozen specimen.