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Test Code LAB478 Lupus Anticoagulant Screen (DRVVT)

Department

HEMATOLOGY

Collection Requirements

Specimen must arrive in the lab within 15 minutes.

Blue top tubes, must be a full draw.

Mix thoroughly after collection.

Primary Collection Container

4 – Blue 2.7 mL (Citrate)

Transport

Platelet-poor plasma frozen

Preferred Transport Temperature

Frozen

Processing

- Specimen must be removed from cells within 30 minutes of collection.

- Mix tubes thoroughly before centrifugation.

- Centrifuge at 3300 rpm for 20 minutes.

- Pour 4mL plasma in to 4 plastic transport vials containing 1 mL each and fast freeze.

Stability

Ambient: Unacceptable

Refrigerated: 4 hours

Frozen: 30 days

Minimum Testing Volume

1.0 mL

Reference Range

Normal

Methodology

Turbidimetric Clot Detection

Dilute Russell Viper Venom Time (DRVVT)

ACL Top

Performed

Monday, Wednesday, and Friday

7:00 am – 2:00 pm

Reported

As completed

CPT Codes

85613; Add 85610, 85730, 85613, and 85390 if reflexed; Additional CPT/charges added as needed to confirm/exclude LAC

Unacceptable Conditions

Hemolyzed, clotted, short draw, non-frozen and serum specimens.

Alternate Collection Container

Lavender 4 mL (K3EDTA)

Additional Information

Lupus Anticoagulants belong to the group of antiphospholipid antibodies which are directed against negatively charged phospholipids or complexes between phospholipids and proteins (either B-2-glycoprotein I, or clotting factors such as prothrombin). When determined by their ability to prolong phospholipid dependent assays (aPTT, dRVVT, SCT), these antibodies are referred to as Lupus Anticoagulant or Lupus-like Inhibitors. Patients with Lupus Anticoagulant are at increased risk of clinical complications such as thrombosis and recurrent miscarriage.