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Test Code LAB50 Bilirubin, Total

Department

CHEMISTRY

Collection Requirements

PROTECT FROM LIGHT.

Primary Collection Container

Green PST (Lithium Heparin)

Alternate Collection Container

Green Microtainer PST

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Separate from cells if testing is delayed more then 8 hours. Deliver to chemistry ASAP.

Stability

Refrigerate: 48 hours, when protected from light and separated from cells.

Minimum Testing Volume

0.1 mL

Reference Range

0-1 Day <5.1 mg/dL

1-2 Days <9.1 mg/dL

2-5 Days <11.1 mg/dL

5-7 Days <9.1 mg/dL

7-15 Days <9.1 mg/dL

>15 Days <1.3 mg/dL

Methodology

Diazotized sulfanilic acid is formed by combining sodium nitrite and sulfanilic acid at low pH. Bilirubin (unconjugated) in the sample is solubilized by dilution in a mixture of caffeine/benzoate/acetate/EDTA. Upon addition of the diazotized sulfanilic acid, the solubilized bilirubin including conjugated bilirubins (mono and diglucoronides) and the delta form (biliprotein-bilirubin covalently bound to albumin) is converted to diazo-bilirubin, a red chromophore representing the total bilirubin which absorbs at 540 nm and is measured using a bichromatic (540, 700 nm) endpoint technique. Siemens Dimension Vista and ExL.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

82247

Unacceptable Conditions

Gross hemolysis.

Prolonged exposure to light.