Sign in →

Test Code LAB51 Bilirubin, Total - Neonatal

Department

CHEMISTRY

Collection Requirements

ONLY FOR BABIES IN NICU OR OB FLOORS.

PROTECT FROM LIGHT.

Wrap specimen in foil if exposure to light is to be prolonged or unavoidable

Primary Collection Container

Green Microtainer PST

Transport

Refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Deliver to chemistry ASAP.

Stability

Refrigerated: 48 hours, when protected from light and separated from cells.

Minimum Testing Volume

0.1 mL

Reference Range

0-1 DAY <5.5 mg/dL

1-2 DAYS <9.1 mg/dL

2-5 DAYS <11.1 mg/dL

5-7 DAYS <9.1 mg/dL

>7 DAYS <9.1 mg/dL

PREMATURE PATIENT RANGES

0-1 DAY <7.1 mg/dL

1-2 DAYS <11.1 mg/dL

2-5 DAYS <13.1 mg/dL

7-15 DAYS <13.1 mg/dL

Methodology

Diazotized sulfanilic acid is formed by combining sodium nitrite and sulfanilic acid at low pH. Bilirubin (unconjugated) in the sample is solubilized by dilution in a mixture of caffeine/benzoate/acetate/EDTA. Upon addition of the diazotized sulfanilic acid, the solubilized bilirubin including conjugated bilirubins (mono and diglucoronides) and the delta form (biliprotein-bilirubin covalently bound to albumin) is converted to diazo-bilirubin, a red chromophore representing the total bilirubin which absorbs at 540 nm and is measured using a bichromatic (540, 700 nm) endpoint technique. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

82247

Unacceptable Conditions

Gross hemolysis.

Not to be ordered on anyone greater than 15 days old. Prolonged esxposure to light.