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Test Code LAB531 Prolactin

Department

CHEMISTRY

Primary Collection Container

Green PST (Lithium Heparin)

Transport

Plasma refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Centrifuge and refrigerate.

Separate from cells if testing is delayed more than 8 hours.

Stability

Ambient: 8 hours

Refrigerated: 48 hours

Minimum Testing Volume

0.2 mL

Reference Range

Non-pregnant: 4.5-34 ng/mL

Pregnant: 12.2-235.9 ng/mL Post Menopause: 3.4-24 ng/mL Male: 3.0-21.1 ng/mL

Methodology

The PRL method is a homogeneous, sandwich chemiluminescent immunoassay based on LOCI® technology. The LOCI® reagents include two synthetic bead reagents and a biotinylated anti-PRL monoclonal antibody fragment. The first bead reagent (Chemibeads) is coated with anti-PRL monoclonal antibody and contains chemiluminescent dye. The second bead reagent (Sensibeads) is coated with streptavidin and contains a photosensitizer dye. Sample is incubated with biotinylated antibody and Chemibeads to form bead-PRL-biotinylated antibody sandwiches. Sensibeads are added and bind to the biotin to form bead-pair immunocomplexes. Illumination of the complex at 680 nm generates singlet oxygen from Sensibeads which diffuses into the Chemibeads triggering a chemiluminescent reaction. The resulting chemiluminescent signal is measured at 612 nm and is a direct function of the prolactin concentration in the sample. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

84146

Unacceptable Conditions

Gross hemolysis.