Test Code LAB96 Lactate Dehydrogenase (LDH)
Department
CHEMISTRY
Primary Collection Container
Green PST (Lithium Heparin)
Alternate Collection Container
Green Microtainer PST
Transport
Plasma or serum refrigerated
Preferred Transport Temperature
Refrigerated
Processing
Specimen should be centrifuged and separated from red cells as soon as possible.
Stability
Ambient: 24 hours
Refrigerated: 48 hours
Frozen: Not recommended
Minimum Testing Volume
0.1 ml plasma
Reference Range
Male reference range
<5 years 181-369 U/L 5-10 yrs 199-353 U/L 10-15 yrs 154-315 U/L >15 yrs 134-241 U/L Female reference range <5 years 184-357 U/L 5-10 yrs 184-320 U/L 10-15 yrs 162-289 U/L >15 yrs 134-241 U/LMethodology
The LDI method uses as a substrate L-lactate buffered at a pH of 9.4. Lactate dehydrogenase oxidizes the substrate in the presence of NAD+ to yield pyruvate and NADH which absorbs light at 340 nm. Lactate dehydrogenase activity is measured as a rate reaction at 340/700 nm, proportional to the amount of lactate dehydrogenase in the sample. Siemens Dimension Vista.
Performed
Sunday – Saturday
Reported
As completed
CPT Codes
83615
Unacceptable Conditions
Hemolysis.
Prolonged contact with cells in excess of 2 hours is unacceptable.