Sign in →

Test Code LAB96 Lactate Dehydrogenase (LDH)

Department

CHEMISTRY

Primary Collection Container

Green PST (Lithium Heparin)

Alternate Collection Container

Green Microtainer PST

Transport

Plasma or serum refrigerated

Preferred Transport Temperature

Refrigerated

Processing

Specimen should be centrifuged and separated from red cells as soon as possible.

Stability

Ambient: 24 hours

Refrigerated: 48 hours

Frozen: Not recommended

Minimum Testing Volume

0.1 ml plasma

Reference Range

Male reference range

<5 years 181-369 U/L 5-10 yrs 199-353 U/L 10-15 yrs 154-315 U/L >15 yrs 134-241 U/L Female reference range <5 years 184-357 U/L 5-10 yrs 184-320 U/L 10-15 yrs 162-289 U/L >15 yrs 134-241 U/L

Methodology

The LDI method uses as a substrate L-lactate buffered at a pH of 9.4. Lactate dehydrogenase oxidizes the substrate in the presence of NAD+ to yield pyruvate and NADH which absorbs light at 340 nm. Lactate dehydrogenase activity is measured as a rate reaction at 340/700 nm, proportional to the amount of lactate dehydrogenase in the sample. Siemens Dimension Vista.

Performed

Sunday – Saturday

Reported

As completed

CPT Codes

83615

Unacceptable Conditions

Hemolysis.

Prolonged contact with cells in excess of 2 hours is unacceptable.