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Test Code MISC1WARDEMTXS Methotrexate

Department

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Reference Lab Test Number

MTXS

Collection Requirements

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Refer To

Warde Medical Laboratory

Primary Collection Container

Red (No Gel)

Alternate Collection Container

Lavender 6 mL (K3EDTA)

Green (Sodium Heparin)

Transport

Serum or plasma frozen

Preferred Transport Temperature

Frozen

Processing

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Centrifuge, separate and send 3.0 mL serum or plasma.

Freeze in an amber screw-capped vial.

Stability

Ambient: 4 hours

Refrigerated: 14 days

Frozen: 6 months

Minimum Testing Volume

1.0 mL

Reference Range

Therapeutic Range:

Low dose:   0.50-1.00 μmol/L
High dose/24 hours:   5.00 μmol/L or less
48 hours:   0.50 μmol/L or less
72 hours:   0.10 μmol/L or less

Methodology

Quantitative Immunoassay

Performed

Sunday – Saturday

Reported

1-2 days

CPT Codes

-80299

Unacceptable Conditions

Serum separator tube (SST)