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Test Code MISC1WARDEUBKQN BK Virus DNA PCR, Quantitative - Urine

Department

SEND OUTS

Reference Lab Test Number

UBKQN

Collection Requirements

Collect random urine.

Dedicated specimens are required. Specimens used in other assays will not be tested.

Primary Collection Container

Sterile Container

Transport

Undiluted urine frozen

Preferred Transport Temperature

Frozen

Processing

Send 2.0 mL urine undiluted, and frozen in a sterile IATA-approved screw-capped plastic container.

Stability

Ambient: 8 hours

Refrigerated: 3 days

Frozen (-20°C): 30 days

Frozen (-70°C): 6 months

Minimum Testing Volume

1.0 mL

Reference Range

BKV QUAL: Not Detected (NOT DET)

BKV QUANT: <250 copies/mL

Log BKV: <2.40 log(10) copies/mL

Methodology

This test utilizes a polymerase chain reaction to amplify sequences from the VP1 regions of the BK virus genome. Real-time detection & quantification are used to determine the viral copy number. The analytical measurement range is 125 to 5 million copies/mL with an extended range up to 50 billion copies/mL with dilution (2.10 to 10.70 log(10 copies/mL). The qualitative limit to detection is 100 copies/mL (2.00 log(10) copies/mL). Specimens reported as “DETECTED” byt <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A “Not detected” result does not rule out infection.

Performed

Monday – Friday

Reported

4 days

CPT Codes

87799

Unacceptable Conditions

Specimens in any gel-based separation media.

Shared speicmens .

Specimens submitted to repeated freeze-thaw cycles. Urine in Grey-top (boric acid) urine Vacutainers. Specimens received in non-sterile or leaking containers.