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Test Code MISC2MAYO7AC4 7AC4, Bile Acid Synthesis, Serum

Important Note

Miscellaneous Test

Specimen Required

Patient Preparation:

1. Patient must be fasting for at least 12 hours; fasting morning specimen is preferred.

2. Patient should not be taking bile acid sequestrants or statins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge and aliquot 1 mL of serum into plastic vial.

2. Send specimen frozen.

Secondary ID


Useful For

Screening for bile acid malabsorption in patients with irritable bowel syndrome-diarrhea (IBS-D)

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

7AC4, Bile Acid Synthesis, S

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 90 days
  Refrigerated  72 hours
  Ambient  24 hours

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

≥18 years: 2.5-63.2 ng/mL

Reference values have not been established for patients who are <18 years of age.

Day(s) and Time(s) Performed

Wednesday; 8 a.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
7AC4 7AC4, Bile Acid Synthesis, S 94866-1


Result ID Test Result Name Result LOINC Value
65504 7AC4, Bile Acid Synthesis, S 94866-1


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.