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HelpTest Code MISC2MAYOABIDR Antibody Identification, Blood and Serum
Shipping Instructions
Specimen must arrive within 72 hours of collection.
Specimen Required
Both blood and serum are required.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Specimen Type: Plasma/Blood
Collection Container/Tube: 6-mL Pink-top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume:
3 mL of plasma
3 mL of red blood cells (RBC)
Collection Instructions:
1. Centrifuge and aliquot plasma into plastic vial.
2. Label specimen as EDTA plasma or EDTA RBC, as appropriate.
3. Send both tubes.
Specimen Type: Serum/Blood
Collection Container/Tube: 10-mL Red top
Submission Container/Tube: Plastic vial
Specimen Volume:
5 mL of serum
5 mL of RBC
Collection Instructions:
1. Centrifuge and aliquot serum into plastic vial.
2. Label specimen as serum or clotted RBC, as appropriate.
3. Send both tubes.
Secondary ID
113389Useful For
Assessing positive pretransfusion antibody screens, transfusion reactions, hemolytic disease of the newborn, and autoimmune hemolytic anemias
This test is not useful for monitoring the efficacy of Rh-immune globulin administration.
This test is not useful for identifying antibodies detected only at 4° C or only after extended room temperature incubation.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| STTX26 | Antibody Panel | No, (Bill Only) | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DCTR | Direct Antiglobulin Test (Poly) | Yes | No |
| SPAGR | Special Red Cell Ag Typing | Yes | No |
| ABTIR | Antibody Titer, RBC | Yes | No |
| STTX25 | Antibody Elution | No, (Bill Only) | No |
| STTX31 | Antibody Adsorption | No, (Bill Only) | No |
| STTX32 | Red Cell Antigen Typing | No, (Bill Only) | No |
Testing Algorithm
The following tests may be ordered and performed as needed for antibody identification: direct antiglobulin testing (polyspecific), including its reflex tests and special red cell antigen typing. Additional reflex charges will be added as needed for antibody elution, antibody adsorption, antibody panels, and special red cell antigen typings.
If certain antibodies are detected and the patient is known to be pregnant, an antibody titration will be performed at an additional charge.
Method Name
Hemagglutination
Reporting Name
Antibody Identification, RBCSpecimen Type
VariesSpecimen Minimum Volume
Blood: 6 mL in EDTA
Pediatric: 2 mL of serum
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | 4 days | |
| Refrigerated | 4 days |
Reject Due To
| Gross hemolysis | OK |
Clinical Information
After exposure to foreign red blood cells (RBC) via transfusion or pregnancy, some people form antibodies that are capable of the destruction of transfused RBC or of fetal RBC in utero. It is important to identify the antibody specificity in order to assess the antibody's capability of causing clinical harm and, if necessary, to avoid the antigen on transfused RBC.
Autoantibodies react against the patient's own RBC as well as the majority of cells tested. Autoantibodies can be clinically benign or can hemolyze the patient's own RBC, such as in cold agglutinin disease or autoimmune hemolytic anemia.
Reference Values
Negative,
If positive, antibodies will be identified and corresponding special red cell antigen typing on patient’s red blood cells will be performed.Â
Interpretation
Specificity of alloantibodies will be stated.
The patient's red blood cells will be typed for absence of the corresponding antigens or as an aid to identification in complex cases.
A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.
Method Description
A panel of reagent type O erythrocytes, with known antigenic determinants and the patient's cells are tested with the patient's serum/plasma. This panel should yield a distinct pattern of agglutination or hemolysis that identifies the auto- or alloantibody specificity. Elution, absorption, neutralization, and other special techniques may be necessary to complete antibody identification.(Fung MK, Eder AF, Spitalnik SL, Westhoff CM. Technical Manual. 19th ed. AABB; 2017)
Day(s) Performed
Monday through Friday, Sunday
Report Available
1 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86870
86860-(if appropriate)
86886-(if appropriate)
86880 x 3 (if appropriate)
86905-(if appropriate)
86978-(if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ABIDR | Antibody Identification, RBC | 888-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ABDR1 | Antibody Identification, RBC | 888-8 |