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Test Code MISC2MAYOHELIS Helicobacter pylori Culture with Antimicrobial Susceptibilities, Varies

Useful For

Recovery of Helicobacter pylori from gastric specimens for antimicrobial susceptibility testing of the organism (amoxicillin, ciprofloxacin, clarithromycin, metronidazole and tetracycline are routinely tested)

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
TISSR Tissue Processing No, (BIll Only) No
MIC Sensitivity, MIC No, (Bill Only) No
SUS Susceptibility No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

 

When Helicobacter pylori is isolated, identification will be confirmed and susceptibility testing performed. The routine susceptibility panel includes amoxicillin, ciprofloxacin, clarithromycin, metronidazole, and tetracycline.

 

See Helicobacter pylori Diagnostic Algorithm in Special Instructions.

Reporting Name

Helicobacter pylori Culture + Susc

Specimen Type

Varies


Shipping Instructions


Specimen must be received in laboratory within 48 hours of collection. Specimen should be collected and packaged as close to shipping time as possible.



Necessary Information


Specimen source is required; include the specific anatomic source.



Specimen Required


Preferred:

Specimen Type: Gastric biopsy

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: Acquire biopsied tissue; moisten with sterile saline.

 

Acceptable:

Specimen Type: Gastric brushings or gastric aspirate

Container/Tube: Sterile container

Specimen Volume: Entire collection


Specimen Minimum Volume

0.5 mL or 0.5 × 0.2 × 0.2-cm sized piece of tissue

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 48 hours

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Biopsy submitted in fluid other than sterile saline

Reference Values

No growth after 7 days

Susceptibility results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as "clinical breakpoints") are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.

 

In some instances an interpretive category cannot be provided based on available data and the following comment will be included: "There are no established interpretive guidelines for agents reported without interpretations."

 

Susceptible (S):

A category defined by a breakpoint that implies that isolates with an MIC at or below the susceptible breakpoint are inhibited by the usually achievable concentrations of antimicrobial agent when the dosage recommended to treat the site of infection is used, resulting in likely clinical efficacy.

Intermediate (I):

A category defined by a breakpoint that includes isolates with MICs within the intermediate range that approach usually attainable blood and tissue levels and for which response rates may be lower than for susceptible isolates.

Note: The intermediate category implies clinical efficacy in body sites where the drugs are physiologically concentrated or when a higher than normal dosage of a drug can be used. This category also includes a buffer zone, which should prevent small, uncontrolled, technical factors from causing major discrepancies in interpretations, especially for drugs with narrow pharmacotoxicity margins.

Resistant (R):

A category defined by a breakpoint that implies that isolates with an MIC at or above the resistant breakpoint are not inhibited by the usually achievable concentrations of the agent with normal dosage schedules and/or that demonstrate MICs that fall in the range in which specific microbial resistance mechanisms are likely, and clinical efficacy of the agent against the isolate has not been reliably shown in treatment studies.(Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing. 29th Informational Supplement. CLSI Supplement M100. Wayne, PA, 2019)

Day(s) and Time(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87081-Helicobacter pylori culture

87077-Bacteria identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87176-Tissue processing (if appropriate)

87181-Susceptibility (if appropriate)

87186-Sensitivity, MIC (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HELIS Helicobacter pylori Culture + Susc 587-6

 

Result ID Test Result Name Result LOINC Value
HELIS Helicobacter pylori Culture + Susc 587-6

Method Name

Conventional Culture Techniques

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Client Test Request (T728)

Secondary ID

62769