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Test Code MISC2MAYOJAK2V JAK2 V617F Mutation Detection, Varies

Reporting Name

JAK2 V617F Mutation Detection, V

Useful For

Aiding in the distinction between a reactive blood cytosis and a chronic myeloproliferative disorder in extracted DNA specimens

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Specimen Required

Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL tube with indication of volume and concentration of the DNA

Specimen Volume: Entire specimen

Collection Instructions: Label specimen as extracted DNA from blood or bone marrow and indicate volume and concentration of the DNA.

Specimen Stability Information: Refrigerated/Ambient

Specimen Minimum Volume

Extracted DNA from blood or bone marrow: 50 microliter at 20 ng/microliter

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; 12 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81270-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder) gene analysis, p.Val617Phe (V617F) variant

LOINC Code Information

Test ID Test Order Name Order LOINC Value
JAK2V JAK2 V617F Mutation Detection, V 43399-5


Result ID Test Result Name Result LOINC Value
39724 JAK2 Result 53761-3
31160 JAK2 V617F Mutation Detection, V 43399-5

Reject Due To

Other Bone marrow biopsies, slides, paraffin shavings Frozen tissues and paraffin-embedded tissues Paraffin-embedded bone marrow aspirates Moderately to severely clotted

Method Name

Point Mutation Detection in DNA Using Quantitative Polymerase Chain Reaction (PCR)


1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.