Test Code MISC2MAYONGMEM Red Blood Cell Membrane Panel, Next-Generation Sequencing, Varies
Secondary ID
64938Useful For
Providing a comprehensive genetic evaluation for patients with a personal or family history suggestive of an RBC membrane disorder
Second-tier testing for patients in whom previous targeted gene mutation analyses were negative for a specific RBC membrane disorder
Establishing a diagnosis of a hereditary RBC membrane disorder, allowing for appropriate management and surveillance of disease features based on the gene involved, especially if splenectomy is a consideration (4)
Identifying mutations within genes associated with phenotypic severity, allowing for predictive testing and further genetic counseling
Special Instructions
Method Name
Next-Generation Sequencing (NGS)
Reporting Name
RBC Membrane Sequencing, VSpecimen Type
VariesShipping Instructions
Peripheral blood specimens must arrive within 30 days of collection.
Necessary Information
1. Metabolic Hematology Next-Generation Sequencing (NGS) Patient Information is required, see Special Instructions. Testing may proceed without the patient information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.
2. If form not provided, include the following information with the test request: clinical diagnosis, pertinent clinical history (ie, CBC results and relevant clinical notes) and differentials based on clinical or morphologic presentation.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Peripheral blood (Preferred)
Container/Tube:
Preferred: Lavender top (EDTA) or Yellow top (ACD)
Acceptable: Green top (heparin)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
3. Label specimen as blood.
Specimen Stability: Refrigerated ≤30 days
Specimen Type: Extracted DNA
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. Indicate volume and concentration of the DNA.
2. Label specimen as extracted DNA and source of specimen.
Specimen Stability: Frozen/Refrigerated/Ambient ≤30 days
Specimen Minimum Volume
Blood: 1 mL
Extracted DNA: 100 mcL at 20 ng/mcL
concentration
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Varies |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Bone marrow biopsies, slides, paraffin shavings Frozen tissues Paraffin-embedded tissues Paraffin-embedded bone marrow aspirates | Reject |
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Monday
Performing Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
81364
81405
81479
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
NGMEM | RBC Membrane Sequencing, V | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
NGMES | Specimen Type | 31208-2 |
NGMED | Indication for Test | 42349-1 |
40568 | Alterations Detected | 82939-0 |
40569 | Interpretation | 59465-5 |
40570 | Additional Notes | 48767-8 |
40571 | Method Summary | 49549-9 |
40572 | Disclaimer | 62364-5 |
40574 | Panel Gene List | 36908-2 |
40575 | Reviewed By | 18771-6 |
Forms
Metabolic Hematology Next-Generation Sequencing (NGS) Patient Information is required, see Special Instructions.
Testing Algorithm
See NGHHA and Subpanel Comparison Gene List in Special Instructions