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Test Code MISC2MAYOP40NA p40 + Napsin A Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Secondary ID

70526

Useful For

p40 aids in the classification of carcinomas and lymphomas

 

Napsin A aids in the identification of primary lung adenocarcinoma

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHMTO IHC Multiplex, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

p40 + Napsin A IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Tissue/Other Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

p40 is an antibody (detected by the chromogen 3,3'-diaminobenzidine [DAB]) that recognizes the deltaNp63 isoform of p63. This isoform may exert an oncogenic effect and is selectively expressed in squamous cell carcinoma. Napsin A is an aspartic proteinase involved in the proteolytic processing of surfactant precursors in the normal alveolar epithelium. In normal tissues, napsin A is expressed in the cytoplasm of alveolar macrophages, type II pneumocytes, pancreatic ducts and acini, and in renal tubules (detected by the chromogen fast red). Napsin A has clinical utility for the identification of primary lung adenocarcinomas. Napsin A is also positive in a subset of thyroid and renal cell carcinomas (especially papillary types).

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Method Description

Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88344-TC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
P40NA p40 + Napsin A IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
70838 p40 + Napsin A IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.