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Test Code MISC2MAYOPPAP Parental Sample Prep for Prenatal Microarray Testing, Blood

Useful For

Preparing parental blood specimen for possible confirmation testing if an abnormality is detected on the prenatal array sample

 

DNA extraction of the maternal blood specimen used for maternal cell contamination testing

Testing Algorithm

This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.

 

When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result is performed on the parental specimens. A paternal blood specimen is requested but not required.

 

Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.

Method Name

DNA Extraction/Cell Culture

Reporting Name

Parental Prenatal Array Prep Test

Specimen Type

Whole blood


Additional Testing Requirements


This test must be ordered in conjunction with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling. Each parent should have a specimen collected under this test ID using a different order number than the fetal specimen collected for the CMAP order.



Necessary Information


1. The reason for testing is required.

2. Include the name listed on the prenatal specimen that was submitted for CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling.



Specimen Required


This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA

 

Specimen Type: Whole blood

Container/Tube: Green top (sodium heparin) and lavender top (EDTA)

Specimen Volume: 3 mL EDTA and 4 mL sodium heparin

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimens in original tubes. Do not aliquot.


Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred)
  Refrigerated 

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Clinical Information

In order to interpret equivocal array results on a prenatal sample (amniotic fluid or chorionic villus), parental studies are performed to determine if the abnormality detected on the prenatal array is inherited or de novo.

 

Maternal cell contamination testing is performed on the maternal blood and prenatal specimen to detect the presence of maternal cells in the fetal sample.

Reference Values

An interpretive report will be provided.

Interpretation

No interpretation will be provided. This test is for specimen processing only.

Method Description

The heparinized sample will be cultured following standard laboratory protocols and a fixed-cell pellet will be prepared. DNA will be extracted from the EDTA sample and used to perform maternal cell contamination testing on the maternal sample. The fixed cell pellet and DNA will be stored in the laboratory for possible confirmation testing if an abnormality is detected on the prenatal array sample.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

This test ID contains no charge and serves as a way to correlate proband parental specimens. If additional testing is warranted, the appropriate tests will be added.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PPAP Parental Prenatal Array Prep Test In Process

 

Result ID Test Result Name Result LOINC Value
55079 Result Summary 50397-9
52978 Interpretation 69965-2
52979 Reason For Referral 42349-1
53337 Specimen 31208-2
53338 Source 31208-2
53339 Method 85069-3

Secondary ID

52964

Specimen Minimum Volume

2 mL EDTA and 2 mL sodium heparin whole blood