Home
HelpTest Code MISC2MAYORBCS Relative B-Cell Subset Analysis Percentage, Blood
Useful For
Screening for humoral or combined immunodeficiencies, including common variable immunodeficiency (CVID), hyper IgM syndrome, among others, where B-cell subset distribution information is desired
Assessing B-cell subset reconstitution after hematopoietic cell (HCT) or bone marrow transplant
Assessing B-cell subset reconstitution following recovery of B cells after B-cell-depleting immunotherapy
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CVID | CVID Confirmation Flow Panel | Yes | No |
Testing Algorithm
This test should be ordered only when percentages (relative distribution of B cell subsets within the total B-cell population) are needed for the reportable B-cell subsets. If both percentages and absolute counts are needed for the reportable B-cell subsets, order IABCS / B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood.
Method Name
Fluorescent Flow Cytometry
Reporting Name
Relative B Cell Subset Analysis %Specimen Type
Whole Blood EDTAShipping Instructions
It is recommended that specimens arrive within 24 hours of draw.
Necessary Information
Ordering physician's name and phone number are required.
Specimen Required
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume:
≤14 years: 4 mL
>14 years: 10 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label specimen as blood for RBCS / Relative B Cell Subset Analysis Percentage.
Specimen Minimum Volume
≤14 years: 3 mL; >14 years: 5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Refrigerated | 48 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) and Time(s) Performed
Monday through Friday
Specimens are required to be received in the laboratory on weekdays and by 4 p.m. on Friday. No weekend processing.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
86356 x7
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| RBCS | Relative B Cell Subset Analysis % | 90416-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BCD19 | CD19+ % of total Lymphocytes | 8117-4 |
| BCD20 | CD20+ % of total Lymphocytes | 8119-0 |
| BCD27 | CD27+ % of CD19+ B Cells | 89358-6 |
| B27MD | CD27+ IgM+ IgD+ % of CD19+ B Cells | 89352-9 |
| B27N | CD27+ IgM- IgD- % of CD19+ B Cells | 89350-3 |
| B27M | CD27+ IgM+ IgD- % of CD19+ B Cells | 89348-7 |
| BIGM | IgM+ % of CD19+ B Cells | 89346-1 |
| B38MN | CD38+ IgM- % of CD19+ B Cells | 89344-6 |
| B38MP | CD38+ IgM+ % of CD19+ B Cells | 89341-2 |
| B21P | CD21+ % of CD19+ B Cells | 89356-0 |
| B21N | CD21- % of CD19+ B Cells | 89355-2 |
| RBCSI | Interpretation | 69048-7 |