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Test Code MISC2MAYOTCRVB TCR V-Beta Repertoire Analysis by Spectratyping, Blood

Useful For

Assessment of T-cell receptor diversity in various clinical contexts including primary immunodeficiencies, monitoring immune reconstitution posthematopoietic cell transplantation, and temporal assessment of repertoire changes in autoimmune diseases and viral infections

Testing Algorithm

Additional tests that could be ordered include TREC / T-Cell Receptor Excision Circles (TREC) Analysis, Blood; CD4RT / CD4 T-Cell Recent Thymic Emigrants (RTE); TCP / T-Cell Subsets, Naive, Memory, and Activated.

Method Name

Molecular TCR Vb-CDR3 Fragment Length Analysis

Reporting Name

TCR Spectratyping, B

Specimen Type

Whole Blood EDTA


Advisory Information


Mayo Laboratory Director/Consultant approval is required prior to ordering this test in patients >40 years of age.



Shipping Instructions


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of draw.

 

Samples arriving on the weekend and observed holidays may be canceled.



Necessary Information


Ordering physician's name and phone number are required.



Specimen Required


For serial monitoring, it is recommended to perform specimen collection at the same time of day, if possible.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume:

Adults: 10 mL

Pediatrics:

-Preferred volume for >1 year: 3 mL

-Preferred volume for ≤1 year: 1 mL

Collection Instructions: Send specimen in original tube.


Specimen Minimum Volume

Adults: 5 mL
Pediatrics: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 48 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Other Clotted

Reference Values

References values will be provided in the patient report.

Day(s) and Time(s) Performed

Varies

Do not draw specimen after Thursday. Specimen must be received by 10 a.m. on Friday.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81340-TRG (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, polymerase chain reaction)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCRVB TCR Spectratyping, B In Process

 

Result ID Test Result Name Result LOINC Value
36473 Family 2 Diversity Ratio 82501-8
36474 Family 3-1 Diversity Ratio 82500-0
36475 Family 4 Diversity Ratio 82499-5
36476 Family 5 Diversity Ratio 82498-7
36477 Family 6 Diversity Ratio 82497-9
36478 Family 7 Diversity Ratio 82496-1
36479 Family 9 Diversity Ratio 82495-3
36480 Family 10 Diversity Ratio 82494-6
36481 Family 11 Diversity Ratio 82493-8
36482 Family 12 Diversity Ratio 82492-0
36483 Family 13 Diversity Ratio 82491-2
36484 Family 14 Diversity Ratio 82490-4
36485 Family 15 Diversity Ratio 82489-6
36486 Family 16 Diversity Ratio 82488-8
36487 Family 18 Diversity Ratio 82487-0
36488 Family 19 Diversity Ratio 82486-2
36489 Family 20-1 Diversity Ratio 82485-4
36490 Family 24-1 Diversity Ratio 82484-7
36491 Family 25 Diversity Ratio 82483-9
36492 Family 27 Diversity Ratio 82482-1
36493 Family 28 Diversity Ratio 82481-3
36494 Family 29 Diversity Ratio 82480-5
36518 Family 30 Diversity Ratio 82479-7
36495 Interpretation 69047-9
36496 Reviewed by 18771-6

Secondary ID

62930