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Test Code MISC8NEOMOLEGFR EGFR Mutation Analysis

Department

Send Outs

Reference Lab Test Number

MOLEGFR

Collection Requirements

FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.

Fine needle aspirate (FNA): Requisition must note specimen is FNA. FFPE cell blocks are acceptable if pathologist attaches note verifying sample has >30% tumor or abnormal cells (required). Minimum 10^6 cells.

Refer To

NeoGenomics

Transport

Ambient

Preferred Transport Temperature

Ambient

Processing

Use cold pack for transporting block during summer to prevent block from melting. Slides can be packed at room temperature

Reference Range

By Report

Methodology

Molecular

Reported

8 days

CPT Codes

81235

Additional Information

Bi-directional sequencing of exons 18-21 of the EGFR gene for detection of EGFR-activating mutations and TKI resistance mutations (including T790M) in these exons. Tumor enrichment is performed before extraction.

Clinical Significance:

EGFR mutation analysis is recommended in non-small cell lung carcinoma (NSCLC) to detect mutations (commonly L858R and exon 19 deletions) associated with increased sensitivity to EGFR tyrosine kinase inhibitors. Detection of TKI resistance mutations such as T790M in patients being treated with a TKI is useful for planning alternate treatment.